Enviro-Friendly Synthesis of Silver Nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy

doi:10.1007/s40195-014-0025-7

Abstract

Abstract:

In the present study, an enviro-friendly synthesis of silver nanoparticles from Berberis lycium Royle leaf extract and their antibacterial efficacy against five pathogenic bacteria were investigated. This biosynthesis technique is proved to be advantageous over physical and chemical methods as no toxic chemicals are used. The structural and morphological characterization was made by UV–visible spectroscopy, scanning electron microscopy, and transmission electron microscopy. The synthesized nanoparticles were oval, rectangular, and spherical in shape, size ranges from 8 to 100 nm and exhibited an absorption peak at 458 nm. The biosynthesized silver nanoparticles have shown good antibacterial effect toward tested bacteria. It is believed that these biosynthesized silver nanoparticles can play a vital role in nano-based products in future.

Key words: Antibacterial, Berberis lycium, Enviro-friendly, Silver nanoparticles

Ansar Mehmood, Ghulam Murtaza, Tariq Mahmood Bhatti, Rehana Kausar. Enviro-Friendly Synthesis of Silver Nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy[J]. Acta Metallurgica Sinica (English Letters), 2014, 27(1): 75-80.

Figures/Tables 9

Fig. 1 Color of plant extract before and after adding AgNO3

Fig. 2 UV–visible spectroscopy of silver colloidal solution

Fig. 3 SEM micrograph of silver nanoparticles prepared from the leaf extract of B. lycium at ×50,000 a, ×30,000 b magnifications

Fig. 4 EDX spectrum of material containing silver nanoparticles

Table 1 EDX results of the silver nanoparticles

Element	wt%	at.%
C	11.25	20.98
O	50.87	71.20
Ag	32.88	6.82
Cd	5.00	1.00

Fig. 5 TEM images of silver nanoparticles at ×40,000 a, ×200,000 b magnifications

Fig. 6 Zones of inhibition of different concentrations of silver nanoparticles against E. colia, K. pneumoniaeb, P. aeruginosac, Proteus spp. d, respectively

Table 2 Antibacterial activity of silver nanoparticles synthesized from leaf extract of B. lycium

AgNPs concentration (μg/mL)	Zone of inhibition (mm) (mean + Standard error of means)
AgNPs concentration (μg/mL)	E. coli	K. pneumoniae	P. aeruginosa	Proteus spp.
20	12.33 ± 0.33	13 ± 0.58	13.67 ± 0.33	12.33 ± 0.33
10	10.67 ± 0.67	11.67 ± 0.33	12.67 ± 0.67	11.33 ± 0.33
5	9 ± 0.58	10.33 ± 0.33	11.33 ± 0.33	9.67 ± 0.67
Leaf extract	–	–	–	–
AgNO₃	10.67 ± 0.33	12 ± 0.58	11.67 ± 0.33	10.33 ± 0.33

Fig. 7 Graphical representation of activity of leaf extract, silver nanoparticles (20, 10, 5 μg/mL), AgNO3 (1 mol/L) against tested bacteria

References 27

[1]	I. Bhatt, B.N. Tripathi, Chemosphere 82, 308(2011)10.1016/j.chemosphere.2010.10.011
[2]	J.Y. Song, B.S. Kim, Bioprocess Biosyst. Eng. 32, 79(2008)10.1007/s00449-008-0224-6
[3]	J.Y. Song, B.S. Kim, Korean J. Chem. Eng. 25, 808(2008)10.1007/s11814-008-0133-z
[4]	P. Mukherjee, A. Ahmad, D. Mandal, S. Senapati, S.R. Sainkar, M.I. Khan, R. Parischa, P.V. Ajayakumar, M. Alam, R. Kumar, M. Sastry, Nano Lett. 1, 515(2001)10.1021/nl0155274
[5]	I. Sondi, B.S. Sondi, J. Colloid Interface Sci. 275, 177(2004)10.1016/j.jcis.2004.02.012
[6]	X. Chen, H.J. Schluesener, Toxicol. Lett. 176, 1(2008)10.1016/j.toxlet.2007.10.004
[7]	J.P. Chen, J. Investig. Cardiol. 19, 395(2007)
[8]	M. Gericke, A. Pinches, Hydrometallurgy 83, 132(2006)10.1016/j.hydromet.2006.03.019
[9]	P.T. Anastas, J.C. Warner,Green Chemistry: Theory and Practice (Oxford University Press, New York, 1998)
[10]	V. Kattumuri, K. Katti, S. Bhaskaran, E.J. Boote, S.W. Casteel, G.M. Fent, D.J. Robertson, M. Chandrasekhar, R. Kannan, K.V. Katti, Small 3, 333(2007)10.1002/smll.200600427
[11]	G. Zhang, B. Keita, A. Dolbecq, P. Mialane, F. Secheresse, F. Miserques, L. Nadjo, Chem. Mater. 19, 5821(2007)10.1021/cm7020142
[12]	K. Renugadevi, R.V. Aswini, Int. J. Nanomater. Biostruct. 2, 5(2012)
[13]	R.B. Malabadi, N.T. Meti, G.S. Mulgund, K. Nataraja, S.V. Kumar, Res. Plant Biol. 2, 32(2012)
[14]	A. Parveen, A.S. Roy, S. Rao, Int. J. Appl. Biol. Pharm. Technol. 3, 222(2012)
[15]	S. Dinesh, S. Karthikeyan, P. Arumugam, Arch. Appl. Sci. Res. 4, 178(2012)
[16]	M. Bharani, T. Karpagam, B. Varalakshmi, G. Gayathiri, K.L. Priya, Int. J. Appl. Biol. Pharm. Technol. 3, 58(2012)
[17]	E.J. Mary, L. Inbathamizh, Asian J. Pharm. Clin. Res. 5, 159(2012)
[18]	P. Sood, R. Modgil, M. Sood, Indian J. Pharm. Biol. Res. 1, 1(2012)
[19]	V. Arya, S. Yadav, S. Kumar, J.P. Yadav, Life Sci. Med. Res. 9, 1(2010)
[20]	M. Zargar, A.A. Hamid, F.A. Bakar, M.N. Shamsudin, K. Shameli, F. Jahanshiri, F. Farahani, Molecules 16, 6667(2011)10.3390/molecules16086667
[21]	N. Ahmad, S. Sharma, V.N. Singh, S.F. Shamsi, A. Fatma, B.R. Mehta, Biotechnol. Res. Int. 2010, 1(2010)
[22]	R. Sarkar, P. Kumbhahar, A.K. Mitra, Dig. J. Nanomater. Biostruct. 5, 419(2010)
[23]	M.N. Priya, B.K. Selvi, J.A.J. Paul, Dig. J. Nanomater. Biostruct. 6, 869(2011)
[24]	P. Magudapatty, P. Gangopadhyayrans, B.K. Panigrahi, K.G.M. Nair, S. Dhara, Physica B 299, 142(2001)10.1016/S0921-4526(00)00580-9
[25]	G. Singhal, R. Bhavesh, K. Kasariya, A.R. Sharma, R.P. Singh, J. Nanopart. Res. 13, 1(2011)10.1007/s11051-010-0193-y
[26]	M. Danilcauk, A. Lund, J. Saldo, H. Yamada, J. Michalik, Spectrochim. Acta A 63, 189(2006)10.1016/j.saa.2005.05.002
[27]	J.S. Kim, E. Kuk, K. Yu, J.H. Kim, S.J. Park, H.J. Lee, S.H. Kim, Y.K. Park, Y.H. Park, C.Y. Hwang, Y.K. Kim, Y.S. Lee, D.H. Jeong, M.H. Cho, Nanomedicine 3, 95(2007)10.1016/j.nano.2006.12.001