Rauvolfia serpentina-Mediated Green Synthesis of CuO Nanoparticles and Its Multidisciplinary Studies

doi:10.1007/s40195-015-0304-y

Abstract

Abstract:

Copper oxide nanoparticles (CuO Nps) were successfully synthesized by solution combustion method using aqueous leaf extract of Rauvolfia serpentina as a fuel. The structure and morphology of the CuO nanoparticles (Nps) were characterized by powder X-ray diffraction (PXRD), UV-visible spectroscopy (UV-visible), scanning electron microscopy (SEM), transmission electron microscopy (TEM), etc. The PXRD patterns reveal the formation of monoclinic phase with crystallite structure. SEM images indicate that the particles have sponge-like structure being highly porous and agglomerated with large surface area. The average crystallite sizes were found to be in the range of 10-20 nm by Scherrer’s method. The CuO Nps size was further confirmed by TEM. Further, CuO Nps exhibit good photocatalytic activity for the photodegradation of trypan blue dye, indicating that it acts as a promising semiconducting material. The antibacterial properties of CuO nanoparticles were investigated against pathogenic bacterial strains, namely Gram -ve Escherichia coli (NCIM-5051) and Pseudomonas desmolyticum (NCIM-2028) and Gram +ve bacteria Staphylococcus aureus (NCIM-5022) using the agar well diffusion method.

Key words: CuO, Nanoparticles, Rauvolfia serpentine, Photocatalytic activity, Antibacterial activity

K. Lingaraju, H. Raja Naika, K. Manjunath, G. Nagaraju, D. Suresh, H. Nagabhushana. Rauvolfia serpentina-Mediated Green Synthesis of CuO Nanoparticles and Its Multidisciplinary Studies[J]. Acta Metallurgica Sinica (English Letters), 2015, 28(9): 1134-1140.

Figures/Tables 8

Fig. 1 PXRD pattern of the synthesized CuO Nps.

Fig. 2 UV-Vis spectrum of the synthesized CuO Nps.

Fig. 3 SEM images a, b& TEM image c of the synthesized CuO Nps.

Fig. 4 Photodegradation of trypan blue as a function of irradiation time under different experimental conditions: a Trypan blue concentration varies from 5 to 25 µg/L, 125 W/m2 UV light, catalyst CuO Nps load 0.5 g, pH value 10; b catalyst CuO Nps load varies from 0.1 to 0.7 g, trypan blue concentration 5 µg/L, 125 W/m2 UV light, pH value 10; c pH value varies from 2 to 12, trypan blue concentration 5 µg/L, 125 W/m2 UV light, catalyst CuO Nps load 0.5 g; d under 125 W/m2 UV and 750 W/m2 sunlight irradiation, 100 mL trypan blue, catalyst CuO Nps load is 0.1 g, and pH value is 10.

Fig. 5 Detection of hydroxyl radicals with different irradiation time.

Fig. 6 Zone of inhibition of antibacterial activity of CuO Nps against: a E. coli; b S. aureus; c P.desmolyticum.

Fig. 7 Graphical representation of antibacterial activity.

Table 1 Antibacterial activity of CuO Nps on pathogenic bacterial strains

Treatment (µg/µL)	E. coli (mm)	S. aureus (mm)	P. desmolyticum (mm)
Standard 5	15.83 ± 0.03*	13.03 ± 0.16	14.13 ± 0.09**
CuO Nps 500	10.10 ± 0.06**	3.17 ± 0.12	2.17 ± 0.03*
CuO Nps 1000	12.13 ± 0.09**	5.90 ± 0.01**	4.13 ± 0.09**

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