Enviro-Friendly Synthesis of Silver Nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy

doi:10.1007/s40195-014-0025-7

金属学报英文版 ›› 2014, Vol. 27 ›› Issue (1): 75-80.DOI: 10.1007/s40195-014-0025-7

收稿日期:2013-06-21 修回日期:2013-07-27 出版日期:2014-02-25 发布日期:2014-03-11

Enviro-Friendly Synthesis of Silver Nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy

Ansar Mehmood¹(), Ghulam Murtaza¹, Tariq Mahmood Bhatti², Rehana Kausar¹

1. Department of Botany, University of Azad Jammu and Kashmir (UAJK), Muzaffarabad-13100, Pakistan
2. Department of Chemical and Material Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad-45650, Pakistan

Received:2013-06-21 Revised:2013-07-27 Online:2014-02-25 Published:2014-03-11

摘要/Abstract

Abstract:

In the present study, an enviro-friendly synthesis of silver nanoparticles from Berberis lycium Royle leaf extract and their antibacterial efficacy against five pathogenic bacteria were investigated. This biosynthesis technique is proved to be advantageous over physical and chemical methods as no toxic chemicals are used. The structural and morphological characterization was made by UV–visible spectroscopy, scanning electron microscopy, and transmission electron microscopy. The synthesized nanoparticles were oval, rectangular, and spherical in shape, size ranges from 8 to 100 nm and exhibited an absorption peak at 458 nm. The biosynthesized silver nanoparticles have shown good antibacterial effect toward tested bacteria. It is believed that these biosynthesized silver nanoparticles can play a vital role in nano-based products in future.

Key words: Antibacterial, Berberis lycium, Enviro-friendly, Silver nanoparticles

. [J]. 金属学报英文版, 2014, 27(1): 75-80.

Ansar Mehmood, Ghulam Murtaza, Tariq Mahmood Bhatti, Rehana Kausar. Enviro-Friendly Synthesis of Silver Nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy[J]. Acta Metallurgica Sinica (English Letters), 2014, 27(1): 75-80.

图/表 9

Fig. 1 Color of plant extract before and after adding AgNO3

Fig. 2 UV–visible spectroscopy of silver colloidal solution

Fig. 3 SEM micrograph of silver nanoparticles prepared from the leaf extract of B. lycium at ×50,000 a, ×30,000 b magnifications

Fig. 4 EDX spectrum of material containing silver nanoparticles

Table 1 EDX results of the silver nanoparticles

Element	wt%	at.%
C	11.25	20.98
O	50.87	71.20
Ag	32.88	6.82
Cd	5.00	1.00

Fig. 5 TEM images of silver nanoparticles at ×40,000 a, ×200,000 b magnifications

Fig. 6 Zones of inhibition of different concentrations of silver nanoparticles against E. colia, K. pneumoniaeb, P. aeruginosac, Proteus spp. d, respectively

Table 2 Antibacterial activity of silver nanoparticles synthesized from leaf extract of B. lycium

AgNPs concentration (μg/mL)	Zone of inhibition (mm) (mean + Standard error of means)
AgNPs concentration (μg/mL)	E. coli	K. pneumoniae	P. aeruginosa	Proteus spp.
20	12.33 ± 0.33	13 ± 0.58	13.67 ± 0.33	12.33 ± 0.33
10	10.67 ± 0.67	11.67 ± 0.33	12.67 ± 0.67	11.33 ± 0.33
5	9 ± 0.58	10.33 ± 0.33	11.33 ± 0.33	9.67 ± 0.67
Leaf extract	–	–	–	–
AgNO₃	10.67 ± 0.33	12 ± 0.58	11.67 ± 0.33	10.33 ± 0.33

Fig. 7 Graphical representation of activity of leaf extract, silver nanoparticles (20, 10, 5 μg/mL), AgNO3 (1 mol/L) against tested bacteria

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Enviro-Friendly Synthesis of Silver Nanoparticles Using Berberis lycium Leaf Extract and Their Antibacterial Efficacy

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